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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • High-content screening (Confocal and Wide-field)
  • Super-resolution imaging (STORM, STED, SIM and AiryScan)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Lattice Light Sheet Microscopy (LLSM)

Highly Multiplexed Imaging

  • Array Tomography
  • Akoya CODEX system (up to 50 biomarkers)

Atomic Force Microscopy

  • Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation/freeze substitution processing
  • High-Pressure Freezing (w/ opto and electric stimulation)
  • Immuno-EM
  • Correlative LM to EM: CLEM
  • Negative staining
  • Ultramicrotomy, cryo and plastic
  • Electron tomography (plastic sections)

Scanning Electron Microscopy

  • High resolution FE-SEM
  • Immuno-SEM
  • Array tomography SEM, CLEM

Bioimage analysis

  • Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)

     

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: the SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland, CSIF Director).
  • Proper Citation: Stanford University Cell Sciences Imaging Core Facility (RRID:SCR_017787)
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    Beckman:

    Shriram:

     

    Events

    July 05, 2022 to July 19, 2022

    Monday, July 18th, 2022
    8:30am - 4:00pm

    Virtual event Signup Link https://gambhir.stanford.edu/

    "This year's event will be fully virtual, but we will offer an in-person watch party of the virtual event for local participants at Stanford Hospital - Assembly Hall. The livestream link will be posted on the website closer to the event."

    July 05, 2022 to July 05, 2023

    The 2 Main Electron Microscopy Techniques: SEM vs TEM
    Published April 6, 2022

    https://bitesizebio.com/29197/electron-microscopy-techniques/?vgo_ee=VLJmq66tCwQ5aNfnxCJBqLTV8qsFUfI%2F1ISxnX2Ui4c%3D

    July 05, 2022

    Scanning Electron Microscopy: 6 SEM Sample Preparation Pointers for Successful Imaging
    Published December 8, 2021

    https://bitesizebio.com/34150/sem-sample-prep/?vgo_ee=VLJmq66tCwQ5aNfnxCJBqLTV8qsFUfI%2F1ISxnX2Ui4c%3D

    June 16, 2022

    Each workshop takes 1 day, in two parts.  *

    Stanford Faculty, Postdocs, and Staff - $100 per workshop (Staff may use STAP funds)

    https://icme.stanford.edu/icme-summer-workshops-2022-fundamentals-data-science

    June 24, 2022 to September 09, 2022

    The Imaging team at CZI is excited to announce the launch of two new RFAs

    The *Advancing Imaging Through Collaborative Projects* is aimed at accelerating the dissemination and adoption of imaging technologies, methods, platforms, or training resources. The deadline to submit applications is Sep. 8.
    https://chanzuckerberg.com/rfa/collaborative-projects-imaging/

    The *Dynamic Imaging RFA* hopes to advance technology directed at real-time visualization of biological processes at the level of cells and molecules.
    Deadline - Aug. 23.
    https://chanzuckerberg.com/rfa/dynamic-imaging/

    For questions, please contact sciencegrants@chanzuckerberg.com Please spread the word to those who may be interested in applying!