The Cell Sciences Imaging Facility (CSIF) is a Beckman Center and Stanford Cancer Institute supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials. The CSIF operates three sites at Stanford University: the SOM Beckman Center CSIF, the WuTsai Neuroscience NMS and the SOE Shriram Center CSIF. These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland and Gordon Wang, CSIF co-Directors).
Technologies Offered
Light Microscopy
- Confocal microscopy (scanning and spinning-disk)
- Two-photon microscopy, Deep tissue imaging objectives
- Wide-field fluorescence microscopy
- Digital deconvolution
- Transmitted-light imaging (phase, DIC, histology)
- High-content screening (Confocal and Wide-field, Apotome)
- Super-resolution (Lattice SIM, SIM, PALM/STORM and AiryScan)
- Cell surface imaging with <100 nM z-resolution (TIRF)
- Specialized microscopy (FRAP, FRET, FLIP...)
- Fluorescence lifetime imaging microscopy (FLIM)
- Lattice Light Sheet Microscopy (LLSM)
- Light Sheet Microscopy (LSM)
Spatial Proteomics - Highly Multiplexed Imaging
- Array Tomography
- Akoya PhenoCycler Fusion and PhenoCycler (CODEX) services (50+ biomarkers)
Atomic Force Microscopy
- Bruker BioScope Resolve integrated onto a Zeiss LSM900/AiryScan2
Events
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Imaris 10.2 updated, Adobe Creative Cloud Available!
AI segmentation in Imaris, Latest Adobe product Access-
News
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CSIF and NMS merge with new co-directors for the unified CSIF
Starting November 1st 2024 the NMS has merged with the CSIF. Gordon Wang and Jon Mulholland are now co-directors of the CSIF.
November 08, 2024
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October 23, 2024
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CSIF Lunch and Learn 10/21 Monday: BC43 Benchtop Microscope and Imaris 10.2 pipeline by Oxford Instruments
CSIF Beckman B050 demo after lunch & learn 10/21 11:30am
September 27, 2024
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April 29, 2024