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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • High-content screening (Confocal and Wide-field)
  • Super-resolution imaging (STORM, STED, SIM and AiryScan)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FCS, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Image Analysis expertise
  • Software for image analysis, processing, and deconvolution
    • Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution

Atomic Force Microscopy

  • Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation processing
  • immuno-EM
  • Correlative LM to EM: CELM
  • negative staining
  • ultramicrotomy, cryo and plastic
  • electron tomography (plastic sections)

Scanning Electron Microscopy

  • variable pressure/environmental
  • high resolution FE-SEM
  • Immuno-SEM
  • array tomography SEM, CLEM

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported, university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of the facility Director).

 

Events

October 01, 2018
Leica Microsystems recently launched powerful new modules for the DMi8 S imaging system - Infinity TIRF and Infinity Scanner - that open up your lab to imaging dynamic processes at the cell surface, as well as a variety of Photomanipulation tools. Total Internal Reflection Fluorescence microscopy is the method of choice to visualize single molecules with super-resolution by maximizing the fluorescent signal-to-noise ratio. This new tool delivers versatile application possibilities with simultaneous multi-color EPI, Hi-Lo and TIRF illumination capability, as well as a high power illumination option for super-resolution applications. In addition, our Infinity Scanner module enables a host of photomanipulation techniques, like FRAP, FLIP, photo activation as well as photo switching, optogenetics and ablation, to help advance your research by combining several photo manipulation modes in a single experiment.
 
Leica Microsystems will be hosting a workshop to introduce the Stanford research community to the DMi8 S with Infinity TIRF and Scanner during the week of October 1. We will open with a Lunch and Learn Seminar followed by Live Demonstrations during the week of October 1.
 
SEMINAR:
Monday, 10/1/18, 12:00 - 1:00 PM
Clark Center
Room S360
Lunch will be provided - Please RSVP at the link below
 
 
OPEN DEMONSTRATIONS:
10/1/18 - 10/5/18
Hosted by the Meyer Lab
Clark Center, Room W229
 
Please contact Olga Davydenko (olga.davydenko@leica-microsystems.com) to reserve hands-on time on the microscope.”

 

October 04, 2018
The Cell Sciences Imaging Facility presents a special seminar:
 
The Antibody Crisis: Leveraging machine learning for evidence-based antibody search
 
The“reproducibility crisis” has generated much attention in the research community over the past years. While the issue is multifaceted at its core, rogue antibodies have been identified as one of the major culprits.
 
To ensure scientists can find antibodies that have been proven to work repeatedly by peers, we developed an open-access resource that uses a machine learning algorithm to screen the literature and identify which and how antibodies have been cited. The resulting peer-reviewed data are searchable by protein targets or product identifier, and are filterable by experimental contexts as cited in papers, including technique, tissue, cell line, to help users pinpoint antibodies that have been published under experimental conditions matching their study interest. 
 
Freely accessible resource for Stanford scientists at https://landing.benchsci.com/academic
 
Time & Date:
Thursday, Oct 4th, 1-2pm
 
Location:
Y2E2 299
 
*Coffee and cookies will be provided