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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • Super-resolution imaging (STORM, STED and SIM)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FCS, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Software for image analysis, processing, and presentation
    • Imaris, Volocity, SVI Huygens, SoftWoRx

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation processing
  • immuno-EM
  • Correlative LM to EM: CELM
  • negative staining
  • ultramicrotomy, cryo and plastic
  • electron tomography (plastic sections)

Scanning Electron Microscopy

  • variable pressure/environmental
  • high resolution FE-SEM
  • Immuno-SEM
  • array tomography SEM, CLEM

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported, university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of the facility Director).



Tomographic Holographic Microscopy Workshop, May 8, 2015
Friday, May 8, 2015 - 09:00

Ever wonder how your living cells will look in 3D, without staining?

The Shriram Center CSIF in Stanford will be hosting the new tomographic holographic microscope from Nanolive on Friday, May 8.

The 3D Cell Explorer is a high speed, high resolution, non-invasive microscope that is able to look inside living cells. This allows you to record stunning, high resolution 3D images of entire cells in just a few seconds.

CONTACT: Cedric Espenel,