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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • High-content screening (Confocal and Wide-field)
  • Super-resolution imaging (STORM, STED, SIM and AiryScan)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Lattice Light Sheet Microscopy (LLSM)

Highly Multiplexed Imaging

  • Array Tomography
  • Akoya CODEX system (up to 50 biomarkers)

Atomic Force Microscopy

  • Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation/freeze substitution processing
  • High-Pressure Freezing (w/ opto and electric stimulation)
  • Immuno-EM
  • Correlative LM to EM: CLEM
  • Negative staining
  • Ultramicrotomy, cryo and plastic
  • Electron tomography (plastic sections)

Scanning Electron Microscopy

  • High resolution FE-SEM
  • Immuno-SEM
  • Array tomography SEM, CLEM

Bioimage analysis

  • Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)

     

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: the SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland, CSIF Director).
  • Proper Citation: Stanford University Cell Sciences Imaging Core Facility (RRID:SCR_017787)
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    Beckman:

    Shriram:

     

    Events

    November 22, 2021 to November 22, 2022

    Check out Imaris homeschool here: https://imaris.oxinst.com/homeschool

    Latest 9.8 release feature highlights: https://imaris.oxinst.com/newrelease

    Contact CSIF kamanl@stanford.edu for remote access info!

    November 30, 2021

    Register Here: https://www.leica-microsystems.com/introduction-to-ultramicrotomy-techniques-and-applications/

    Leica VIRTUAL WORKSHOP:  Introduction to Ultramicrotomy Techniques and Applications
    Tuesday, December 21, 2021   10AM PST / 12:00CST
    Presenter: Andrew Lawson, Advanced Workflow Specialist, Leica Microsystems
    Ultramicrotomy is a widely adopted and versatile technique which can be utilized to prepare samples for a range of characterization methods. It is often used in biological workflows to prepare ultrathin sections for TEM, as well as semi-thin sections and flat surfaces for light microscopy and other bulk characterization methods. In materials science, ultramicrotomy is often used to prepare polymeric and even hard metals and ceramics for similar characterization workflows.
    In this presentation, we will introduce some basic techniques, considerations, and applications for ultramicrotomy workflows. We will cover both biological and materials ultramicrotomy and show some application examples for both areas. We will also show a live demonstration of the complete sectioning workflow utilizing the Leica EM UC7* to prepare and collect ultrathin sections of a resin-embedded biological sample.

     

    January 03, 2022 to January 17, 2022

    1/18-1/20/22 UC Berkeley 

    Free signup for this virtual event: https://hopin.com/events/advanced-imaging-methods-workshop-2022/registration

    This year's conference will focus on:

    Time-correlated Single Photon Counting
    Lightsheet advances
    Optogenetics and neurobiology
    Open Science
    Artificial Intelligence and Image Analysis
    Quality & Reproducibility in Imaging
    Tutorials for beginners and advanced microscopy users from academic and industry experts

    January 07, 2022 to February 15, 2022

    Aesthetically pleasing microscopy image contest, one click submission, why not!

    Submissions are open for the first annual Andrew Olson Scientific Image Contest.

    Open to all Stanford researchers (and not limited to neuroscience). Submit your most aesthetically amazing scientific art by Feb 15, 2022: http://neuroscience.stanford.edu/scientific-image-awards

    November 15, 2021 to December 15, 2021

    CSIF will be hosting an instrument introductory webinar on 12/24 Wednesday 10am PST titled

    "Reveal the vibrant sub-organelle network of life with ZEISS Elyra 7 with Lattice SIM²"

    Please signup if you're interested in joining here: https://www.zeiss.com/microscopy/us/local/workshops/2021/elyra-7-roadshow/stanford.html  Further in-person demo can be arranged 11/29-10/10.  Please inquire with Kitty at kamanl@stanford.edu or Zeiss Rep benjamin.figueroa@zeiss.com for details.