Cell Sciences Imaging Facility (CSIF)
Yuanyuan Li (CSIF) : Highly-multiplexed tonsil tissue imaged with Phenocycler Fusion
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The Cell Sciences Imaging Facility (CSIF) is a Beckman Center and Stanford Cancer Institute supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials. The CSIF operates four sites at Stanford University: the SOM Beckman Center CSIF, the SOE Shriram Center CSIF, the SRP CSIF (to open door in Fall 2026 at 3176 Porter Dr room 161/158) , and the WuTsai Neuroscience NMS . These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Gordon Wang, CSIF Director).
Confocal. Judith Maria Kraiczy, Human Fallopian Tube Cells
Highly-Multiplexed TMA - Phenocycler (Yuanyuan Li, CSIF)
Transmission Electron Microscopy. Fly Brain 1200x (John Perrino, CSIF)
Scanning Electron Microscopy. Cilia mouse trachea
Tiled Confocal Image. Mouse lung.
Fluorescence Life Time Microscopy (FLIM). FLIM image of mouse cerebellum purkinje cells
Technologies Offered
Light Microscopy
- Confocal microscopy (scanning and spinning-disk)
- Two-photon microscopy, Deep tissue imaging objectives
- Wide-field fluorescence microscopy
- Digital deconvolution
- Transmitted-light imaging (phase, DIC, histology)
- High-content screening (Confocal and Wide-field, Apotome)
- Super-resolution (Lattice SIM, SIM, PALM/STORM and AiryScan)
- Cell surface imaging with <100 nM z-resolution (TIRF)
- Specialized microscopy (FRAP, FRET, FLIP...)
- Fluorescence lifetime imaging microscopy (FLIM)
- Lattice Light Sheet Microscopy (LLSM)
- Light Sheet Microscopy (LSM)
Spatial-Omics - Highly Multiplexed Imaging
- Akoya PhenoCycler Fusion and PhenoCycler (CODEX) services (50+ biomarkers)
- 3D Spatial Transcriptomics in Thick Tissue Up To 100 µm (STARmap)
Atomic Force Microscopy
- Bruker NanoWizard V
- Bruker BioScope Resolve integrated onto a Zeiss LSM900/AiryScan2
Locations
