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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • High-content screening (Confocal and Wide-field)
  • Super-resolution imaging (STORM, STED, SIM and AiryScan)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FCS, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)

Highly Multiplexed Imaging (up to 50 biomarkers)

  • Akoya CODEX system

Atomic Force Microscopy

  • Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation/freeze substitution processing
  • High-Pressure Freezing (w/ opto and electric stimulation)
  • Immuno-EM
  • Correlative LM to EM: CELM
  • Negative staining
  • Ultramicrotomy, cryo and plastic
  • Electron tomography (plastic sections)

Scanning Electron Microscopy

  • Variable pressure/environmental
  • High resolution FE-SEM
  • Immuno-SEM
  • Array tomography SEM, CLEM

Bioimage analysis

  • Develop image analysis workflow for specific project (ImageJ, Python)
  • Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)

     

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of the facility Director).

 

Events

October 24, 2019

What the SCOPE! Imaging Seminar Series

The Cell Sciences Imaging Facility (CSIF), a Beckman Service Center, will be hosting a seminar series starting with:

Jon Mulholland, Director CSIF: Overview of CSIF resources

Anum Khan, Staff Scientist:

Highly multiplex tissue imaging: CODEX

Date/Time: Thursday, October 24 @ 3pm (networking reception to follow)

Location: Beckman Center, Munzer Auditorium

The series begins with an overview of imaging technologies available in the facility, followed by an in-depth presentation of the new, highly multiplex tissue imaging and analysis service: CODEX.

The seminar series is open and free to the Stanford community.

Learn more and register: LINK