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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • High-content screening (Confocal and Wide-field)
  • Super-resolution imaging (STORM, STED, SIM and AiryScan)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Lattice Light Sheet Microscopy (LLSM)

Highly Multiplexed Imaging

  • Array Tomography
  • Akoya CODEX system (up to 50 biomarkers)

Atomic Force Microscopy

  • Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation/freeze substitution processing
  • High-Pressure Freezing (w/ opto and electric stimulation)
  • Immuno-EM
  • Correlative LM to EM: CLEM
  • Negative staining
  • Ultramicrotomy, cryo and plastic
  • Electron tomography (plastic sections)

Scanning Electron Microscopy

  • High resolution FE-SEM
  • Immuno-SEM
  • Array tomography SEM, CLEM

Bioimage analysis

  • Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)

     

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: the SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland, CSIF Director).
  • Proper Citation: Stanford University Cell Sciences Imaging Core Facility (RRID:SCR_017787)
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    Beckman:

    Shriram:

     

    Events

    November 22, 2021 to June 22, 2022

    Stay updated on our Huygens Pro software: https://youtu.be/dbLmeGs25Js

    Visit svi.nl for full details.

    Contact CSIF kamanl@stanford.edu for remote access info!

    November 22, 2021 to November 22, 2022

    Check out Imaris homeschool here: https://imaris.oxinst.com/homeschool

    Latest 9.8 release feature highlights: https://imaris.oxinst.com/newrelease

    Contact CSIF kamanl@stanford.edu for remote access info!

    November 15, 2021 to December 15, 2021

    CSIF will be hosting an instrument introductory webinar on 12/24 Wednesday 10am PST titled

    "Reveal the vibrant sub-organelle network of life with ZEISS Elyra 7 with Lattice SIM²"

    Please signup if you're interested in joining here: https://www.zeiss.com/microscopy/us/local/workshops/2021/elyra-7-roadshow/stanford.html  Further in-person demo can be arranged 11/29-10/10.  Please inquire with Kitty at kamanl@stanford.edu or Zeiss Rep benjamin.figueroa@zeiss.com for details.

    February 18, 2021

    Bitplane's Imaris will be hosting Online Workshop for Stanford Users 2/23, please register and contact Lynsey.Hamilton@bitplane.com about details.

    September 09, 2020

    Recordings available upon request.