Cell Sciences Imaging Facility (CSIF)

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center and Stanford Cancer Institute supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials. The CSIF operates three sites at Stanford University: the SOM Beckman Center CSIF, the WuTsai Neuroscience NMS and the SOE Shriram Center CSIF. These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland and Gordon Wang, CSIF co-Directors).

Confocal. Judith Maria Kraiczy, Human Fallopian Tube Cells

Highly-Multiplexed TMA - Phenocycler (Yuanyuan Li, CSIF)

Transmission Electron Microscopy. Fly Brain 1200x (John Perrino, CSIF)

Scanning Electron Microscopy. Cilia mouse trachea

Tiled Confocal Image. Mouse lung.

Fluorescence Life Time Microscopy (FLIM). FLIM image of mouse cerebellum purkinje cells

Technologies Offered

Light Microscopy
- Confocal microscopy (scanning and spinning-disk)
- Two-photon microscopy, Deep tissue imaging objectives
- Wide-field fluorescence microscopy
- Digital deconvolution
- Transmitted-light imaging (phase, DIC, histology)
- High-content screening (Confocal and Wide-field, Apotome)
- Super-resolution (Lattice SIM, SIM, PALM/STORM and AiryScan)
- Cell surface imaging with <100 nM z-resolution (TIRF)
- Specialized microscopy (FRAP, FRET, FLIP...)
- Fluorescence lifetime imaging microscopy (FLIM)
- Lattice Light Sheet Microscopy (LLSM)
- Light Sheet Microscopy (LSM)
Spatial Proteomics - Highly Multiplexed Imaging
- Array Tomography
- Akoya PhenoCycler Fusion and PhenoCycler (CODEX) services (50+ biomarkers)
Atomic Force Microscopy
- Bruker NanoWizard V
- Bruker BioScope Resolve integrated onto a Zeiss LSM900/AiryScan2

Electron Microscopy
- Transmission Electron Microscopy (biological)
  - Chemical and cryo-fixation/freeze substitution processing
  - High-Pressure Freezing (w/ opto and electric stimulation)
  - Immuno-EM
  - Correlative LM to EM: CLEM
  - Negative staining
  - Ultramicrotomy
  - Electron tomography (plastic sections)
- Scanning Electron Microscopy
  - High resolution FE-SEM
  - Immuno-SEM
  - Array tomography SEM, CLEM
Bioimage analysis
- Provide software with extra modules geared for batch image corrections and analysis, real-time processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx, Microvolution)
- VisioPharm software with Phenoplex : complete image analysis workflow for multiplexed image analysis needs in spatial biology.