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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • High-content screening (Confocal and Wide-field)
  • Super-resolution imaging (STORM, STED, SIM and AiryScan)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FCS, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)

Highly Multiplexed Imaging (up to 50 biomarkers)

  • Akoya CODEX system

Atomic Force Microscopy

  • Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation/freeze substitution processing
  • High-Pressure Freezing (w/ opto and electric stimulation)
  • Immuno-EM
  • Correlative LM to EM: CELM
  • Negative staining
  • Ultramicrotomy, cryo and plastic
  • Electron tomography (plastic sections)

Scanning Electron Microscopy

  • Variable pressure/environmental
  • High resolution FE-SEM
  • Immuno-SEM
  • Array tomography SEM, CLEM

Bioimage analysis

  • Develop image analysis workflow for specific project (ImageJ, Python)
  • Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)

     

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of the facility Director).

 

Events

The Cell Sciences Imaging Facility's next seminar series will feature BioImage Processing and Analysis:

Cedric Espenel, CSIF Staff Scientist

Date/Time: Thursday, February 27 @ 3pm (networking reception (food and drinks!) to follow)

Location: Beckman Center, Munzer Auditorium

The Cell Sciences Imaging Facility (CSIF), a Beckman Service Center, aims to illustrate how bioimage analysis methods can answer defined biological questions.



Imaging technologies are improving rapidly and the ability to routinely acquire large multi-dimensional datasets is revolutionizing biomedical research. In order to fully extract and derive biological meaning from the vast quantity of information that is potentially available in these datasets, automated analysis pipelines are needed, but many life scientists lack the necessary expertise to design and execute such analyses.


The key objectives of the seminar are:

  •     Briefly introduce biomedical researchers to bioimage analysis
  •     Inform about the possibilities that image analysis can bring to their research
  •     Showcase some of the analysis pipelines that were developed at CSIF
  •     Establish links between life science researchers and image analysis experts

The seminar series is open and free to the Stanford community.

Learn more and register: LINK