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Yuanyuan Li (CSIF) : Highly-multiplexed tonsil tissue imaged with Phenocycler Fusion
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The Cell Sciences Imaging Facility (CSIF) is a Beckman Center  and Stanford Cancer Institute supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: the SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland, CSIF Director).

Technologies Offered

  • Light Microscopy

    • Confocal microscopy (scanning and spinning-disk)
    • Two-photon microscopy
    • Wide-field fluorescence microscopy
    • Digital deconvolution
    • Transmitted-light imaging (phase, DIC, histology)
    • High-content screening (Confocal and Wide-field)
    • Super-resolution imaging (SIM and AiryScan)
    • Cell surface imaging with <100 nM z-resolution (TIRF)
    • Specialized microscopy (FRAP, FRET, FLIP...)
    • Fluorescence lifetime imaging microscopy (FLIM)
    • Lattice Light Sheet Microscopy (LLSM)
    • Light Sheet Microscopy (LSM)
  • Spatial Proteomics - Highly Multiplexed Imaging

    • Array Tomography
    • Akoya PhenoCycler Fusion and PhenoCycler (CODEX) services (50+ biomarkers)
  • Atomic Force Microscopy

    • Bruker BioScope Resolve integrated onto a Zeiss LSM900/AiryScan2
  • Electron Microscopy

    • Transmission Electron Microscopy (biological)

      • Chemical and cryo-fixation/freeze substitution processing
      • High-Pressure Freezing (w/ opto and electric stimulation)
      • Immuno-EM
      • Correlative LM to EM: CLEM
      • Negative staining
      • Ultramicrotomy
      • Electron tomography (plastic sections)
    • Scanning Electron Microscopy

      • High resolution FE-SEM
      • Immuno-SEM
      • Array tomography SEM, CLEM
  • Bioimage analysis

    • Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)