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The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials. The CSIF operates two sites at Stanford University: the SOM Beckman Center CSIF and the SOE Shriram Center CSIF. These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland, CSIF Director).
Confocal Volume. Eva Huang, Dunn Lab, human embryonic stem cells.
Super Resolution Microscopy. BPAE cells, CSIF
Transmission Electron Microscopy. Fly Brain 1200x (John Perrino)
Scanning Electron Microscopy. Cilia mouse trachea
Tiled Confocal Image. Mouse lung.
Fluorescence Life Time Microscopy (FLIM). FLIM image of mouse cerebellum purkinje cells
Technologies Offered
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Light Microscopy
- Confocal microscopy (scanning and spinning-disk)
- Two-photon microscopy
- Wide-field fluorescence microscopy
- Digital deconvolution
- Transmitted-light imaging (phase, DIC, histology)
- High-content screening (Confocal and Wide-field)
- Super-resolution imaging (SIM and AiryScan)
- Cell surface imaging with <100 nM z-resolution (TIRF)
- Specialized microscopy (FRAP, FRET, FLIP...)
- Fluorescence lifetime imaging microscopy (FLIM)
- Lattice Light Sheet Microscopy (LLSM)
- Light Sheet Microscopy (LSM)
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Spatial Proteomics - Highly Multiplexed Imaging
- Array Tomography
- Akoya CODEX system (50+ biomarkers)
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Atomic Force Microscopy
- Bruker BioScope Resolve
Location
