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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • High-content screening (Confocal and Wide-field)
  • Super-resolution imaging (STORM, STED, SIM and AiryScan)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FCS, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Lattice Light Sheet Microscopey (LLSM)

Highly Multiplexed Imaging (up to 50 biomarkers)

  • Akoya CODEX system
  • Array Tomography

Atomic Force Microscopy

  • Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation/freeze substitution processing
  • High-Pressure Freezing (w/ opto and electric stimulation)
  • Immuno-EM
  • Correlative LM to EM: CLEM
  • Negative staining
  • Ultramicrotomy, cryo and plastic
  • Electron tomography (plastic sections)

Scanning Electron Microscopy

  • High resolution FE-SEM
  • Immuno-SEM
  • Array tomography SEM, CLEM

Bioimage analysis

  • Develop image analysis workflow for specific project (ImageJ, Python)
  • Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)

     

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The SOM Beckman Center CSIF and the SOE Shriram Center CSIF.  Both sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland, CSIF Director)

 

Beckman:

Shriram:

Wu Tsai:

SIM 1:

 
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Events

SVI Huygens (deconvolution, image clearing and correction) software fully updated on our virtual machine, with latest versions and features!  Please contact Kitty Lee for remote use instructions.  More about the software package, see link.

May 28, 2020
PLEASE READ ATTACHED DOCUMENT FOR COVID-19 RELATED UPDATE REGARDING CSIF