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Transmission Electron Microscopy

Fly Brain 1200x

Super Resolution Microscopy

BPAE cells, CSIF

Scanning Electron Microscopy

Cilia mouse trachea

Confocal Volume

Eva Huang, Dunn Lab, human embryonic stem cells.

Tiled Confocal Image

Mouse lung

Fluorescence Life Time Microscopy (FLIM)

FLIM image of mouse cerebellum purkinje cells

Technologies Offered:

Light Microscopy

Confocal microscopy (scanning and spinning-disk)
Two-photon microscopy
Wide-field fluorescence microscopy
Digital deconvolution
Transmitted-light imaging (phase, DIC, histology)
High-content screening (Confocal and Wide-field)
Super-resolution imaging (STORM, STED, SIM and AiryScan)
Cell surface imaging with <100 nM z-resolution (TIRF)
Specialized microscopy (FRAP, FRET, FLIP...)
Fluorescence lifetime imaging microscopy (FLIM)
Lattice Light Sheet Microscopy (LLSM)

Highly Multiplexed Imaging

Array Tomography
Akoya CODEX system (up to 50 biomarkers)

Atomic Force Microscopy

Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

Chemical and cryo-fixation/freeze substitution processing
High-Pressure Freezing (w/ opto and electric stimulation)
Immuno-EM
Correlative LM to EM: CLEM
Negative staining
Ultramicrotomy, cryo and plastic
Electron tomography (plastic sections)

Scanning Electron Microscopy

High resolution FE-SEM
Immuno-SEM
Array tomography SEM, CLEM

Bioimage analysis

Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials. The CSIF operates two sites at Stanford University: the SOM Beckman Center CSIF and the SOE Shriram Center CSIF. These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland, CSIF Director).
Proper Citation: Stanford University Cell Sciences Imaging Core Facility (RRID:SCR_017787)

Beckman:

Shriram:

Events

Imaris Virtualization and Analysis Workshop 2/23, consultation 2/23-2/25

February 18, 2021

Bitplane's Imaris will be hosting Online Workshop for Stanford Users 2/23, please register and contact Lynsey.Hamilton@bitplane.com about details.

Aivia BioImage Analysis software webinar Recording available now!

November 11, 2020

Recording of Aivia BioImage Analysis software webinar happened 11/9 online is now available for all to review.

Aside from the expected rendering and neuron tracing, particle trackings kind of quantifications, CSIF finds it a valuable user-friendly tool for users to dabble into machine-teaching/learning, custom pipeline creation.

Go and check it out HERE! Also take advantage of the available topic-oriented workshops and the open-source community.

The software has made available a cloud-computing version that one can request license to test out, meaning you don't need the best computer to handle the rendering.

A new version 9.5 launch will happen 11/18 Wednesday.