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Technologies Offered:

Light Microscopy

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • High-content screening (Confocal and Wide-field)
  • Super-resolution imaging (STORM, STED, SIM and AiryScan)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Specialized microscopy (FRAP, FRET, FLIP...)
  • Fluorescence lifetime imaging microscopy (FLIM)
  • Light Sheet Microscopy (LSM)
  • Lattice Light Sheet Microscopy (LLSM)

Highly Multiplexed Imaging

  • Array Tomography
  • Akoya CODEX system (up to 50 biomarkers)

Atomic Force Microscopy

  • Bruker BioScope Resolve

Electron Microscopy

Transmission Electron Microscopy (biological)

  • Chemical and cryo-fixation/freeze substitution processing
  • High-Pressure Freezing (w/ opto and electric stimulation)
  • Immuno-EM
  • Correlative LM to EM: CLEM
  • Negative staining
  • Ultramicrotomy, cryo and plastic
  • Electron tomography (plastic sections)

Scanning Electron Microscopy

  • High resolution FE-SEM
  • Immuno-SEM
  • Array tomography SEM, CLEM

Bioimage analysis

  • Provide software for image analysis, processing, and deconvolution (Imaris, Volocity, SVI Huygens, SoftWoRx. Microvolution)

     

The Cell Sciences Imaging Facility (CSIF) is a Beckman Center supported university service center that provides high resolution, state-of-the-art light and electron microscopy technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates three sites at Stanford University: The SOM Beckman Center CSIF, the SOE Shriram Center CSIF and the  Wu Tsai Neurosciences Institute Microscopy Service NMS.  These sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval of Jon Mulholland, CSIF Director)

 

Beckman:

Shriram:

Wu Tsai:
 

Events

November 11, 2020

Recording of Aivia BioImage Analysis software webinar happened 11/9 online is now available for all to review.  

Aside from the expected rendering and neuron tracing, particle trackings kind of quantifications, CSIF finds it a valuable user-friendly tool for users to dabble into machine-teaching/learning, custom pipeline creation.   

Go and check it out HERE!  Also take advantage of the available topic-oriented workshops and the open-source community. 

The software has made available a cloud-computing version that one can request license to test out, meaning you don't need the best computer to handle the rendering.  

A new version 9.5 launch will happen 11/18 Wednesday.
September 09, 2020

Recordings available upon request.

SVI Huygens (deconvolution, image clearing and correction) software fully updated on our virtual machine, with latest versions and features!  Please contact Kitty Lee for remote use instructions.  More about the software package, see link.
May 28, 2020
PLEASE READ ATTACHED DOCUMENT FOR COVID-19 RELATED UPDATE REGARDING CSIF