Bruker BioAFM Resolve
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More information about the microscope
AFM Probe price list:
| Probe | Price per tip ($) |
|---|---|
| ScanAsyst Fluid + | 32 |
| ScanAsyst Fluid | 29.5 |
| ScanAsyst Air | 29.5 |
| MLCT-Bio-DC | 35 |
| PFQNM-LC-A-CAL | 100 |
| RTESPA-525 | 38.5 |
| DNP-10 | 25 |
| OBL-10 | 38 |
| RTESPA-300 | 38.5 |
| MSNL-10 | 30 |
| SNL-10 | 27.5 |
| PEAKFORCE-HiRs-F-A | 70 |
| PEAKFORCE-HiRs-F-B | 70 |
| RTESPA-150 | 38.5 |
More information about the probe or if you want to purchase your own.
For Colloidal probes you have to custom order on Novascan.
Cleaning the probe head for AFM
- Prepare a 2% solution of Alconox in MilliQ water (200 mg to 10mL water) in a clean glass vial or 15mL falcon tube
- To clean the head, place it on the secure attachment provided by Bruker.
- Add 2 drops of the Alconox solution on the head (where you will put the probe). Ensure that water is not enough to drip over to the piezo
- Once the drops are on the head, brush it gently using the toothbrush provided in the facility. You should see foam
- At this point, rinse the probe head by adding MilliQ water drop-wise (Full rinsing will consume about 2mL of water). Alternatively, you can make 3 solutions of water in dishes and dip the probe head in them one by one (1 minute dip, followed by the next dish of water)
- Dry the probe head using N2 flow
- Rinse the head with pure 100% EtOH drop-wise. Shake the excess off and rinse again with fresh pure100% EtOH. Repeat (total of 3 times).
- Dry with N2 flow
- The probe head is now ready to be used. In case you still see impurities in your sample, consider using better detergents or longer cleaning of the probe head.
Acknowledgement: We thank Saumya Saurabh for writing the detailed protocol for cleaning probe heads.