Stanford
Cell Sciences Imaging Facility CSIF
(CSIF)
Momei Zhou : varicella-zoster virus replication in human melanoma cells.

Light Microscopes

Instruments

Leica Stellaris8 DIVE upright Confocal, multi-photon

  • Upright laser scanning confocal with 3HyDS, 1HydX detectors, 2 NDDs, transmittance (total of 4Hyds can be used simultaneously)
  • Deep tissue imaging with 16x/0.6 immersion and 20x/1.0 water immersion lens
  • Live cell imaging with excitation including 730nm laser and detection up to 850nm.  Oko-lab environment control setups.
  • Extra large field of view, focus-adapting tile scanning with Navigator
  • FRET/FRAP
  • Tunable Multi-photon DeepSee Maitai Laser 690-1040 nm, second harmonics (SHG), polarizer possible if using HyD.
  • For all data published using this microscope you must acknowledge NIH ORIP funding: 1 S10 OD032300-01

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Location: Beckman Center, Room B050

Zeiss Elyra7 Lattice SIM super resolution with widefield "apotome" 

  • Live Cell Imaging
  • STORM, TIRF, Lattice SIM, SIM, Widefield apotome, leap, burst mode
  •  10x, 20x, 25x, 40x, 63x/1.4, 63x/1.46

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Location: Beckman Center, Room B050A

Inverted Zeiss LSM 880 Laser Scanning Confocal Microscope

  • Laser scanning confocal
  • Live Cell Imaging, Linkam cooling/heating stage
  • Imaging of weak fluorescence signals with high sensitivity AirScan mode
  • Super Resolution imagining with AiryScan (1.7X improvement over diffraction limited imaging)
  • FAST AiryScan (acquisition speeds of 27fps (at 480 x 480 pixels))
  • FRET, FRAP and FLIP
  • Photoactivation/ -conversion
  • Photon Counting

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Location: Beckman Center, Room B050

Leica SP8 inverted Confocal with WLL

  • Supercontinuum White light laser 470-670nm, up to 8 lines
  • 3x gated, hybrid detectors, 2x PMT
  • Spectral Imaging, Unmixing 
  • Excitation Fingerprinting
  • Live Cell Imaging
  • Ultra-fast resonant scanner(8000Hz=30ms bidirectional for 512line frame)
  • FRAP/FLIP/FRET
  • Transmittance detection/DIC/Phase
  • Detector upgraded for 2x scan speed than standard SP8
PeCon POC-R cell cultivation/perfusion setup available (supplies updated 4/2023) (965.78 KB)

 

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Location: Beckman Center, Room B050C

Inverted Zeiss LSM 780 Multiphoton Laser Scanning Confocal Microscope

  • Laser scanning confocal
  • Deep tissue imaging
  • Live Cell Imaging
  • Spectral Imaging, Unmixing
  • Super Resolution imagining with AirScan (1.7X improvement over diffraction limited imaging)
  • Excitation Fingerprinting
  • FLIM
  • FRET and Anisotropy
  • FRAP and FLIP
  • FCS
  • Photoactivation/ -conversion

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Location: Shriram Center, Room B023

Inverted Nikon Crest V3 Spinning Disk Confocal Microscope

The referenced media source is missing and needs to be re-embedded.
  • Live Cell Imaging
  • Fast Confocal Imaging (1000 frames per second possible)
  • High Content Screening and Throughput
  • TIRF (to image events at the cell surface ~100nm)
  • Large Field of View for WideField Imaging (>3200x3200, 6.5 um pixel)
  • Possibly to do near infrared excitation (730nm)
  • FRET

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Location: Shriram Center, Room B023

OMX BLAZE 3D-structured illumination, super-resolution microscope (SIM)

  • Super Resolution Microscopy
  • 3D-Structured Illumination Microscopy (3D-SIM)
  • Stochastic Optical Reconstruction Microscopy (STORM, AF647 only)
  • TIRF
  • 3D Deconvolution
  • Live cell imaging

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Location: Beckman Center, Room B050

Inverted Zeiss AxioObserver Z1 Widefield Microscope

  • sCMOS monochrome and color cameras
  • general-purpose widefield fluorescence microscopy
  • Calibri 7 LED light source
  • image large biological specimen at high resolution (Stitching)
  • Histology imaging (color camera)

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Location: Shriram Center, Room B023

Leica DM2000 histology scope with color cameras

  • Color camera
  • Histology

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Location: Beckman Center, Room B001

 

Bruker Truelive Light Sheet

  • The dual-sided illumination light-sheet microscope
  • Resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.

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Location: Shriram Center, Room B023

ZEISS Lattice Light Sheet 7

  • Long-term volumetric imaging of living cells
  • Fast live cell imaging at subcellular resolution
  • Uses standard sample carriers.

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Location: Shriram Center, Room B023

Nikon Ti-2e inverted widefield microscope

  •  Plan Apo 4x/0.20 WD=20mm dry lens
  •  Plan Apo 20x/0.8 WD=0.8mm air lens (have room to install more! inquire)
  • Sola light source
  • Photometrics Iris15 camera(15Megapixel, 25mm Field of View, 30 Frames/s, 73% QE)
  • Filters: Dapi, Cy3, Cy5, mCherry, Cy7, Brightfield
  •  slide stitching/ scanning and NIS-Elements AR batch JOBS module along with AR analysis module.

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Location: Beckman Center, Room B050C