Light Microscopes

Inverted, laser scanning confocal microscope for imaging from the UV to the near IR, Fluorescence-lifetime, and 2-photon.

•  Point scanning confocal with up to 5 sumultaneous detection channels, from 410 to 850 nm
• Environmental control for live cell imaging (temperature and CO2)
• Excitation and emission wavelength scanning
• Fluorescence lifetime imaging (FLIM)
• Deconvolution for super resolution
• Pulsed infrared laser for 2-photon and Second Harmonic Generation (SHG) imaging
• Förster Resonance Energy Transfer (FRET) by intensity or lifetime methods
• Photobleaching/photoablation (FRAP)
• Multiwell plate imaging

Required acknowledgements: 

Please cite “Stanford University Cell Sciences Imaging Core Facility (RRID:SCR_017787)” and state “The Leica Stellaris confocal was purchased with funding from Stanford University Community of Shared Advanced Research Platforms (C-ShaRP) (RRID:SCR_022986)"

• Live Cell Imaging
• STORM, TIRF, Lattice SIM, SIM, Widefield apotome, leap, burst mode
• 10x, 20x, 25x, 40x, 63x/1.4, 63x/1.46
• Required Acknowledgement:The following acknowledgement is required when publishing data obtained with this instrument: "The project described was supported, in part, by Award Number 1S10OD034400-01A1 from the S10 Instrumentation Programs. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the S10 Instrumentation Programs or the National Institutes of Health."

• Laser scanning confocal
• Live Cell Imaging support with heating stage and CO2
• Imaging of weak fluorescence signals with high sensitivity AirScan mode
• Super Resolution imagining with AiryScan (1.7X improvement over diffraction limited imaging)
• FAST AiryScan (acquisition speeds of 27fps (at 480 x 480 pixels))
• FRET, FRAP and FLIP
• Photoactivation/ -conversion
• Photon Counting

• Supercontinuum White light laser 470-670nm, up to 8 lines
• 3x gated, hybrid detectors, 2x PMT
• Spectral Imaging, Unmixing
• Excitation Fingerprinting
• Live Cell Imaging
• Ultra-fast resonant scanner(8000Hz=30ms bidirectional for 512line frame)
• FRAP/FLIP/FRET
• Transmittance detection/DIC/Phase
• Detector upgraded for 2x scan speed than standard SP8

Inverted, laser scanning confocal microscope for imaging from the UV to the near IR, Fluorescence-lifetime, and 2-photon.

• Point scanning confocal with up to 5 sumultaneous detection channels, from 410 to 850 nm
• Environmental control for live cell imaging (temperature and CO2)
• Excitation and emission wavelength scanning
• Fluorescence lifetime imaging (FLIM)
• Deconvolution for super resolution
• Pulsed infrared laser for 2-photon and Second Harmonic Generation (SHG) imaging
• Förster Resonance Energy Transfer (FRET) by intensity or lifetime methods
• Photobleaching/photoablation (FRAP)
• Multiwell plate imaging

Required acknowledgements: 

Please cite “Stanford University Cell Sciences Imaging Core Facility  (RRID:SCR_017787)” and state “The Leica Stellaris confocal was purchased with funding from Stanford University Community of Shared Advanced Research Platforms (C-ShaRP) (RRID:SCR_022986)"

Reserve this microscope

Location: Cell Sciences Imaging Facility, Shriram Center 023

• Live Cell Imaging
• Fast Confocal Imaging (1000 frames per second possible)
• High Content Screening and Throughput
• TIRF (to image events at the cell surface ~100nm)
• Large Field of View for WideField Imaging (>3200x3200, 6.5 um pixel)
• Possibly to do near infrared excitation (730nm)
• FRET

• Laser scanning confocal
• Live Cell Imaging
• Super Resolution imagining with AirScan2 (32-channel detector) - delivers enhanced sensitivity and super-resolution imaging beyond conventional confocal, with faster acquisition and reduced phototoxicity.)
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Reserve this microscope

Location: Shriram Center, Room B023

• Color camera
• Histology