Light Microscopes
Main content start
Instruments
SP8 WLL
- Supercontinuum White light laser 470-670nm, up to 8 lines
- 3x gated, hybrid detectors
- Spectral Imaging, Unmixing
- Excitation Fingerprinting
- Live Cell Imaging
- Ultra-fast resonant scanner(8000Hz=30ms bidirectional for 512line frame)
- FRAP/FLIP/FRET
- Transmittance detection/DIC/Phase
- Detector upgraded for 2x scan speed than standard SP8
Location: Beckman Center, Room B050C
This microscope was funded, in apart, by Award Number 1S10OD010580 from the National Center for Research Resources (NCRR).
Leica SP8 Stellaris DIVE upright Confocal, multi-photon
|
Location: Beckman Center, Room B050
Inverted Zeiss LSM 880 Laser Scanning Confocal Microscope
- Laser scanning confocal
- Live Cell Imaging,
- Imaging of weak fluorescence signals with high sensitivity AirScan mode
- Super Resolution imagining with AiryScan (1.7X improvement over diffraction limited imaging)
- FAST AiryScan (acquisition speeds of 27fps (at 480 x 480 pixels))
- FRET, FRAP and FLIP
- Photoactivation/ -conversion
- Photon Counting
Location: Beckman Center, Room B050
Inverted Zeiss LSM 780 Multiphoton Laser Scanning Confocal Microscope
- Laser scanning confocal
- Deep tissue imaging
- Live Cell Imaging
- Spectral Imaging, Unmixing
- Super Resolution imagining with AirScan (1.7X improvement over diffraction limited imaging)
- Excitation Fingerprinting
- FLIM
- FRET and Anisotropy
- FRAP and FLIP
- FCS
- Photoactivation/ -conversion
Location: Shriram Center, Room B023
Inverted Nikon Crest V3 Spinning Disk Confocal Microscope
The referenced media source is missing and needs to be re-embedded.
- Live Cell Imaging
- Fast Confocal Imaging (1000 frames per second possible)
- High Content Screening and Throughput
- TIRF (to image events at the cell surface ~100nm)
- Large Field of View for WideField Imaging (>3200x3200, 6.5 um pixel)
- Possibly to do near infrared excitation (730nm)
- FRET
Location: Shriram Center, Room B023
OMX BLAZE 3D-structured illumination, super-resolution microscope (SIM)
- Super Resolution Microscopy
- 3D-Structured Illumination Microscopy (3D-SIM)
- Stochastic Optical Reconstruction Microscopy (STORM, AF647 only)
- TIRF
- 3D Deconvolution
- Live cell imaging
Location: Beckman Center, Room B050
3i Lattice Light Sheet Microscope V2
- Live Cell Imaging
- Low Phototoxicity for Prolonged Living Cell Imaging
Location: Beckman Center, Room B050
Inverted Zeiss AxioObserver Z1 Widefield Microscope
- sCMOS monochrome and color cameras
- general-purpose widefield fluorescence microscopy
- Calibri 7 LED light source
- image large biological specimen at high resolution (Stitching)
- Histology imaging (color camera)
Location: Shriram Center, Room B023
Leica DM2000 histology scope with color cameras
- Color camera
- Histology
Location: Beckman Center, Room B001
Bruker Truelive Light Sheet
- The dual-sided illumination light-sheet microscope
- Resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.
Location: Shriram Center, Room B023
ZEISS Lattice Light Sheet 7
- Long-term volumetric imaging of living cells
- Fast live cell imaging at subcellular resolution
- Uses standard sample carriers.
Location: Shriram Center, Room B023