Light Microscopes

Instruments

Leica Stellaris8 DIVE upright Confocal, multi-photon

Upright laser scanning confocal with 3HyDS, 1HydX detectors, 2 NDDs, transmittance (total of 4Hyds can be used simultaneously) Deep tissue imaging with 16x/0.6 immersion and 20x/1.0 water immersion lens Live cell imaging with excitation including 730nm laser and detection up to 850nm.  Oko-lab environment control setups. Extra large field of view, focus-adapting tile scanning with Navigator FRET/FRAP Tunable Multi-photon DeepSee Maitai Laser 690-1040 nm, second harmonics (SHG), polarizer possible if using HyD. For all data published using this microscope you must acknowledge NIH ORIP funding: 1 S10 OD032300-01

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Location: Beckman Center, Room B050

Zeiss Elyra7 Lattice SIM super resolution with widefield "apotome" 

Live Cell Imaging STORM, TIRF, Lattice SIM, SIM, Widefield apotome, leap, burst mode 10x, 20x, 25x, 40x, 63x/1.4, 63x/1.46 Required Acknowledgement:The following acknowledgement is required when publishing data obtained with this instrument: "The project described was supported, in part, by Award Number 1S10OD034400-01A1 from the S10 Instrumentation Programs. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the S10 Instrumentation Programs or the National Institutes of Health."

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Location: Beckman Center, Room B050A

Inverted Zeiss LSM 880 Laser Scanning Confocal Microscope

Laser scanning confocal Live Cell Imaging, Linkam cooling/heating stage Imaging of weak fluorescence signals with high sensitivity AirScan mode Super Resolution imagining with AiryScan (1.7X improvement over diffraction limited imaging) FAST AiryScan (acquisition speeds of 27fps (at 480 x 480 pixels)) FRET, FRAP and FLIP Photoactivation/ -conversion Photon Counting

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Location: Beckman Center, Room B050

Leica SP8 inverted Confocal with WLL

Supercontinuum White light laser 470-670nm, up to 8 lines 3x gated, hybrid detectors, 2x PMT Spectral Imaging, Unmixing Excitation Fingerprinting Live Cell Imaging Ultra-fast resonant scanner(8000Hz=30ms bidirectional for 512line frame) FRAP/FLIP/FRET Transmittance detection/DIC/Phase Detector upgraded for 2x scan speed than standard SP8

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Location: Beckman Center, Room B050C

Inverted Zeiss LSM 780 Multiphoton Laser Scanning Confocal Microscope

• Laser scanning confocal
• Deep tissue imaging
• Live Cell Imaging
• Spectral Imaging, Unmixing
• Super Resolution imagining with AirScan (1.7X improvement over diffraction limited imaging)
• Excitation Fingerprinting
• FLIM
• FRET and Anisotropy
• FRAP and FLIP
• FCS
• Photoactivation/ -conversion

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Location: Shriram Center, Room B023

Inverted Nikon Crest V3 Spinning Disk Confocal Microscope

• Live Cell Imaging
• Fast Confocal Imaging (1000 frames per second possible)
• High Content Screening and Throughput
• TIRF (to image events at the cell surface ~100nm)
• Large Field of View for WideField Imaging (>3200x3200, 6.5 um pixel)
• Possibly to do near infrared excitation (730nm)
• FRET

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Location: Shriram Center, Room B023

OMX BLAZE 3D-structured illumination, super-resolution microscope (SIM)

• Super Resolution Microscopy
• 3D-Structured Illumination Microscopy (3D-SIM)
• Stochastic Optical Reconstruction Microscopy (STORM, AF647 only)
• TIRF
• 3D Deconvolution
• Live cell imaging

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Location: Beckman Center, Room B050

Inverted Zeiss AxioObserver Z1 Widefield Microscope

• sCMOS monochrome and color cameras
• general-purpose widefield fluorescence microscopy
• Calibri 7 LED light source
• image large biological specimen at high resolution (Stitching)
• Histology imaging (color camera)

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Location: Shriram Center, Room B023

Leica DM2000 histology scope with color cameras

• Color camera
• Histology

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Location: Beckman Center, Room B001

Bruker Truelive Light Sheet

• The dual-sided illumination light-sheet microscope
• Resolution down to 255 nm in xy, enabling resolving subcellular structures in living samples free of phototoxic effects.

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Location: Shriram Center, Room B023

ZEISS Lattice Light Sheet 7

• Long-term volumetric imaging of living cells
• Fast live cell imaging at subcellular resolution
• Uses standard sample carriers.

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Location: Shriram Center, Room B023

Nikon Ti-2e inverted widefield microscope

Plan Apo 4x/0.20 WD=20mm dry lens Plan Apo 20x/0.8 WD=0.8mm dry lens Plan Apo 40x/0.95 WD=0.25-0.17 dry lens Plan Apo 60x/1.42 WD=0.15 oil lens Sola light source Photometrics Iris15 camera(15Megapixel, 25mm Field of View, 30 Frames/s, 73% QE) Filters: Dapi, Cy3, Cy5, mCherry, Cy7, Brightfield  Filter Cubes: EX (nm)   EM (nm)   Dichroic DAPI 341-371 356/30 417-477 447/60 409 GFP/FITC/CY2 446-486 466/40 500-550 525/50 495 CY3/TRITC 532.5-557.5 545/25 570-640 605/70 565 TR/mCherry 542-582 562/40 603.5-678.5 641/75 593 Cy5 626-644 635/18 659-701 680/42 652 CY7 670.5-745.5 708/75 768.5-849.5 809/81 757 slide stitching/ scanning and NIS-Elements AR batch JOBS module along with AR analysis module.

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Location: Beckman Center, Room B050C