Upright Zeiss AxioImager Epifluorescence / Widefield Microscope
Inverted Zeiss Axio Observer Z1 Epifluorescence / Widefield Microscope
The Zeiss Axio Observer is a motorized, inverted microscope equipped for both transmitted light (bright field, phase) and fluorescence microscopy. This microscope is a general-purpose widefield fluorescence microscopy. It is equipped with 2 cameras, a sCMOS for high resolution fluorescence imaging and a color camera that can be used to image histology slides.
System specifications
- Inverted Zeiss AxioObserver Z1
- Piezo-driven XY scanning stage with linear encoders, travel range:225 - 85mm, maximum speed:100mm/s, resolution:0.2um, reproducibility:<1um, absolute accuracy:+/-5um Motorized Z-drive with 10nm resolution
- DC motorized Z-drive with optoelectronic coding, resolution 10nm, reproducibility 10nm
- Calibri7 illumination source, R[G/Y]CBV-UV, coupled to scope with liquid light guide.
- Manual 3-position tube lens: mag.1.0X, mag.1.60X, Bertrand lens
- 12V/100W halogen light source, manual condenser, 0.9NA, brightfield/DIC/phase contrast/
Detection
- Orca Flash4.0 V3 sCMOS Camera
- Axiocam 506 Color
Software
- Zen Blue
Objectives (each with DIC slider)
mag. |
type |
NA |
Working Distance |
10X |
Plan Apo |
0.4 |
5.4 mm |
20X |
Plan Apo |
0.8 |
0.55 mm |
64X (Oil) |
EC Epiplan Neofluar |
1.4 |
0.19 mm |
Fluorescence filter sets:(Semrock Brightline #)
description
|
|
dichroic
|
emission
|
DAPI (1160A) |
387/11 |
409 |
447/60 |
GFP (3035B) |
472/30 |
495 |
520/35 |
FITC (3540B) |
482/35 |
506 |
536/40 |
YFP (2427A) |
500/24 |
520 |
542/27 |
TXRED (4040B) |
562/40 |
593 |
624/40 |
CY5 (4040A) |
628/40 |
660 |
692/40 |
Camera Specifications:
ORCA-Flash4.0 V3 |
|
Sensors |
sCMOS |
CCD Basic resolution |
2048 X 2048 = 4.2 megapixels |
Pixel size |
6.50um (h) x 6.50um (v) |
Sensor size |
Chip area 13.312 mm x 13.312 mm |
Quantum efficiency
|
82% |
Digitization |
High Quality: 16 bit |
CCD cooling |
Single stage Peltier cooling |
Camera Specifications: ZEISS Axiocam 506 color
Sensor |
Sony ICX 694, EXview HAD CCD II |
CCD Basic resolution |
6 Megapixel: 2752 (H) x 2208 (V) |
Pixel size |
4.54 μm x 4.54 μm |
Spectral sensitivity |
Approx. 400 nm − 720 nm, Hoya C5000 / RGB Bayer color filter mask |
Sensor size |
Image diagonal 16 mm, equivalent to 1" sensor format |
Dynamic range |
1:2500 (68 dB) |
Exposure Time Range |
250 μs to 60 s |
Digitalization |
14 Bit / Pixel |
Readout Speed |
39 Mhz, 13 Mhz, switchable readout clock speed |
Readout Noise (typical) |
6.5 e- at 39 Mhz / 6.0 e- at 13 Mhz |