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Inverted Zeiss LSM 780 multiphoton laser scanning confocal microscope

RESERVE THIS MICROSCOPE


The microscope is coupled with a Spectra Physics MaiTai, DeepSee ultrafast pulsed laser system for multi-photon excitation deep within tissue. It is equipped with the LSM BiGmodule which provides two highly sensitive GaAsP detection channels (45% QE compared to ~25% QE for conventional PMT).?It is fully enclosed for heating and environmental control with media perfusion capabilities and CO2 control.

Applications:

  • Deep tissue imaging
  • Imaging of weak fluorescence signals
  • Live Cell Imaging
  • Spectral Imaging, Unmixing
  • Excitation Fingerprinting
  • Super Resolution imagining with AirScan (1.7X improvement over diffraction limited imaging)
  • Ion Imaging
  • FLIM
  • FRET and Anisotropy
  • FRAP and FLIP
  • FCS
  • Photoactivation/ -conversion
  • Photon Counting

System specifications:

  • Inverted Zeiss Axio Observer Z1 microscope with Definite Focus
  • Motorized XY Stage, allowing large images and multi-point time-lapse imaging
  • 34 spectral detection channels via GaAsP photodetector plus two PMTs
  • BiG Module with 2 GaAsP photodetector
  • MaiTai DeepSee?(allow highest pick power)
  • 2-photon microscopy with non-descanned BiG detectors
  • Spectral unmixing, spectral fingerprinting and photon counting
  • InVivo Scientific enclosure w/ environmental control (temperature and CO2)
  • Up to 8 frames/sec with 512 x 512 pixels (max. 250 frames/sec with 512 x 16)
  • Up to 6144 x 6144 pixels resolution
  • Detection from 390 to 750 nm freely configurable

Software:

  • Zen Black
  • Zen Blue

Zen lite free download page here:

http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen-lite.html#downloads

Objectives 

Name

Magnification

NA

Immersion

working distance

EC PLAN NEO

10x

0.3

air

5.2mm

PLAN APO

20x

0.8

air

0.55 mm

EC PLAN NEO

40x

1.3

oil

0.21 mm

C-APO

40x

1.2

water

0.28 mm

PLAN APO

63x

1.4

oil

0.19 mm

LCI PLAN NEO

25x

0.8

Oil, Gly, Water

0.57 mm

Laser lines:

1P lasers:

  • 405nm
  • 458nm
  • 488nm
  • 514nm
  • 561nm
  • 594nm
  • 633nm

Laser Power measure out of the 20X Dry (in nW) - 05/08/2019

Laser Wavelength in nm 1% 10% 100%
405 41 194 1850
458 494 4490 49200
488 50 337 3260
514 37 227 2170
561 208 1930 18300
594 15 32 194
633 24 151 1360

2P laser:

  • Tuning range: 690-1040nm

Detection:

32 anode Hybrid-GaAsP detector for increased light sensitivity/spectra unmixing, 2 standard fluorescent photomultiplier tubes (PMT).

Off-hour credits:

  • Labs that use the following microscopes: Zeiss LSM780 or Nikon-SDC for 25 or more hours per month, during OFF-HOURS will be charged $30/hour for all use of that microscope.
  • Labs that use the Zeiss LSM780 or Nikon SDC, live cell image system 75 or more hours per month, during OFF-HOURS will be charged at $20/hour for usage. This rate is in recognition of the high number of hours required for continuous overnight and weekend imaging inherent in the use of these microscopes for live cell imaging.

To qualify for these rates you must have after-hours access to the facility (i.e. be an experienced, competent user) and must use the confocal/2 photon between 5:00 pm and 8:00 am M-F, or any time on weekends. Reserved but unused time does not qualify for these discounts. Please speak with the facility Director for more information.

Funding:

This microscope was funded by jointly by the SOE and the Beckman Center (Dr. Alex Dunn and Jon Mulholland, PIs). Installation date: 09/2014

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