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Upright Zeiss AxioImager Epifluorescence / Widefield Microscope

Upright Zeiss AxioImager Epifluorescence / Widefield Microscope

The Zeiss AxioImager is a motorized, upright microscope equipped for both transmitted light (DIC, Dark Field, Phase Contrast and Polarization) and fluorescence microscopy. This microscope is equipped for Array Tomography technique, as well as general-purpose widefield fluorescence microscopy.

System specifications:

  • Upright Zeiss AxioImager
  • Piezo-driven XY scanning stage with linear encoders, travel range:225 - 85mm, maximum speed:100mm/s, resolution:0.2um, reproducibility:<1um, absolute accuracy:+/-5um Motorized Z-drive with 10nm resolution
  • DC motorized Z-drive with optoelectronic coding, resolution 10nm, reproducibility 10nm
  • EXFO X-Cite 120 illumination source, coupled to scope with liquid light guide, motorized 10-position reflector turret with DIC cube and fluorescence filter cubes (see below)
  • Manual 3-position tube lens: mag.1.0X, mag.1.60X
  • Bertrand lens 12V/100W halogen light source, manual condenser, 0.9NA, brightfield/DIC/phase contrast/

Detection:

  • ZEISS Axiocam 503 mono
  • ZEISS Axiocam 506 color

Software:

  • Zen pro

Objectives (each with DIC slider)

mag.

type

NA

Working Distance

10X

Plan Apo

0.4

5.4 mm

20X

Plan Apo

0.8

0.55 mm

64X (Oil)

EC Epiplan Neofluar

1.4

0.19 mm

fluorescence filter sets:(Semrock Brightline #)

 

description

 

 

dichroic

 

emission

 

DAPI (1160A)

387/11

409

447/60

GFP (3035B)

472/30

495

520/35

FITC (3540B)

482/35

506

536/40

YFP (2427A)

500/24

520

542/27

TXRED (4040B)

562/40

593

624/40

CY5 (4040A)

628/40

660

692/40

 

Camera Specifications: ZEISS Axiocam 506 mono

Sensor

Sony ICX 694, EXview HAD CCD II

CCD Basic resolution

6 Megapixel: 2752 (H) x 2208(V)

Pixel size

4.54 μm x 4.54 μm

Sensor size

Image diagonal 16 mm, equivalent to 1" sensor format

Dynamic range

1:2500 (68 dB)

Exposure Time Range

250 μs to 60 s

Digitalization

14 Bit / Pixel

Readout Speed

39 Mhz, 13 Mhz, switchable readout clock speed

Readout Noise (typical)

6.5 e- at 39 Mhz / 6.0 e- at 13 Mhz

 

 

Camera Specifications: ZEISS Axiocam 506 color

Sensor

Sony ICX 694, EXview HAD CCD II

CCD Basic resolution

6 Megapixel: 2752 (H) x 2208 (V)

Pixel size

4.54 μm x 4.54 μm

Spectral sensitivity

Approx. 400 nm − 720 nm, Hoya C5000 / RGB Bayer color filter mask

Sensor size

Image diagonal 16 mm, equivalent to 1" sensor format

Dynamic range

1:2500 (68 dB)

Exposure Time Range

250 μs to 60 s

Digitalization

14 Bit / Pixel

Readout Speed

39 Mhz, 13 Mhz, switchable readout clock speed

Readout Noise (typical)

6.5 e- at 39 Mhz / 6.0 e- at 13 Mhz