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Light Microscopes

Instruments

Leica SP8, Whitelight Laser, Lightning, Klondike upgraded, gSTEDClick

  • Supercontinuum White light laser 470-670nm, up to 8 lines
  • 3x gated, hybrid detectors
  • Spectral Imaging, Unmixing 
  • Excitation Fingerprinting
  • Live Cell Imaging
  • gated Stimulated Emission Depletion (gSTED)
  • Ultra-fast resonant scanner(8000Hz=30ms bidirectional for 512line frame)
  • FRAP/FLIP/FRET
  • Transmittance detection/DIC/Phase
  • Klondike upgraded 2x scan speed than standard SP8

RESERVE THIS MICROSCOPE

LOCATION: Beckman Center, Room B050C


 

Leica SP5 upright Confocal, multi-photon, FLIMClick

  • Upright laser scanning confocal with 2HyD detectors
  • Deep tissue imaging with 20x/1.0 water immersion lens
  • Live cell imaging
  • Extra large field of fiew (23mm)
  • FRET/FRAP / FLIP
  • Tunable Multi-photon DeepSee Maitai Laser 700-1000nm
  • Becker&Hickl TCSPC SimpleTau FLIM

RESERVE THIS MICROSCOPE

LOCATION: Beckman Center, Room B050


 

Inverted Zeiss LSM 880 Laser Scanning Confocal MicroscopeClick

  • Laser scanning confocal
  • Live Cell Imaging
  • Imaging of weak fluorescence signals with high sensitivity AirScan mode
  • Super Resolution imagining with AiryScan (1.7X improvement over diffraction limited imaging)
  • FAST AiryScan (acquisition speeds of 27fps (at 480 x 480 pixels))
  • FRET, FRAP and FLIP
  • Photoactivation/ -conversion
  • Photon Counting

RESERVE THIS MICROSCOPE

LOCATION: Beckman Center, Room B050


 

Inverted Zeiss LSM 780 Multiphoton Laser Scanning Confocal MicroscopeClick

  • Laser scanning confocal
  • Deep tissue imaging
  • Live Cell Imaging
  • Spectral Imaging, Unmixing
  • Super Resolution imagining with AirScan (1.7X improvement over diffraction limited imaging)
  • Excitation Fingerprinting
  • FLIM
  • FRET and Anisotropy
  • FRAP and FLIP
  • FCS
  • Photoactivation/ -conversion

RESERVE THIS MICROSCOPE

LOCATION: Shriram Center, Room B023


 

Inverted Zeiss LSM 980 Laser Scanning Confocal MicroscopeClick

  • Laser scanning confocal
  • Fast Live Cell Imaging with Airyscan2 Multiplex (~10fps with zoom 1)
  • Super Resolution imagining with AirScan 2 (1.7X improvement over diffraction limited imaging)
  • Spectral Imaging, Unmixing
  • Excitation Fingerprinting
  • Ion Imaging
  • FRET and Anisotropy
  • FRAP and FLIP
  • Photoactivation/ -conversion

RESERVE THIS MICROSCOPE

LOCATION: Wu Tsai Neuroscience Institute, Room S080


 

Inverted Zeiss LSM 710 Laser Scanning Confocal MicroscopeClick

  • Laser scanning confocal
  • Live Cell Imaging
  • FRAP and FLIP
  • Photoactivation/ conversion

RESERVE THIS MICROSCOPE

LOCATION: Lokey/SIM1, Room G0827


 

Inverted Nikon Spinning Disk Confocal MicroscopeClick

  • Live Cell Imaging
  • Fast Confocal Imaging (>50 frames per second of 2 colors simultaneously)
  • High Content Screening and Throughput
  • TIRF (to image events at the cell surface ~100nm)
  • Large Field of View for WideField Imaging (>2000 x 2000)
  • Possibly to do near infrared excitation (730nm)
  • FRET

RESERVE THIS MICROSCOPE

LOCATION: Shriram Center, Room B023


 

OMX BLAZE 3D-structured illumination, super-resolution microscope (SIM)Click

  • Super Resolution Microscopy
  • 3D-Structured Illumination Microscopy (3D-SIM)
  • Stochastic Optical Reconstruction Microscopy (STORM, AF647 only)
  • TIRF
  • 3D Deconvolution
  • Live cell imaging

RESERVE THIS MICROSCOPE

LOCATION: Beckman Center, Room B050


 

3i Lattice Light Sheet Microscope V2Click

  • Live Cell Imaging
  • Low Phototoxicity for Prolonged Living Cell Imaging

RESERVE THIS MICROSCOPE

LOCATION: Beckman Center, Room B005


 

LaVision Ultramicroscope - Light Sheet Microscope (NMS)Click

  • The microscope is for imaging cleared tissue volumes.
  • 6 bi-directional light sheets: thickness 4–6 μm, width 1–20 mm
  • refractive index matching 1.33–1.56
  • lines available: 405, 488, 561, 639, 785 nm
  • total magnification 1.7–17.6 (objective lens + zoom ratio)(
  • supports both aqueous [e.g. CLARITY] and organic [e.g., iDISCO+] solvents
  • sample travel range (X, Y, Z) 1 cm, 1 cm, 1 cm

RESERVE THIS MICROSCOPE

LOCATION: Wu Tsai Neuroscience Institute, Room S080


 

Upright Zeiss AxioImager Widefield Microscope (Shriram)Click

  • Array Tomography
  • general-purpose widefield fluorescence microscopy
  • image large biological specimen at high resolution (Stitching)
  • Histology imaging

RESERVE THIS MICROSCOPE

LOCATION: Shriram Center, Room B023


 

Upright Zeiss AxioImager Widefield Microscope (NMS)Click

  • Array Tomography
  • general-purpose widefield fluorescence microscopy
  • image large biological specimen at high resolution (Stitching)
  • Histology imaging

RESERVE THIS MICROSCOPE

LOCATION: Wu Tsai Neuroscience Institute, Room S080


 

Leica DM2000 histology scope with color camerasClick

  • Color camera
  • Histology

RESERVE THIS MICROSCOPE

LOCATION: Beckman Center, Room B001