LatticeSIM Microscope
The Zeiss Elyra7 super-resolution microscope (widefield Apotome, laser scanning SIM, Lattice SIM, SMLM, TIRF)(CARL Zeiss Microsystems) can resolve structures down to 60nm, observe live cell dynamics at up to 255 fps, achieve sharpest sectioning in wide-field microscopy, run TIRF and single molecule detection(20-40nm axial resolution), or live, 3D, tiled imaging with realtime 3D deconvolution processing.
The Elyra7 is capable of simultaneous, fast, multi-channel 3D Stripe SIM/lattice SIM super-resolution imaging (tens of microns deep). In addition to the 3D SIM modality, Elyra7 has also been designed for stochastic localization super resolution (PALM/dSTORM/PAINT ~lateral 10-20nm). Additionally, two sensitive detectors (Duolink Hamamatsu ORCA Fusion BT sCMOS qe~95%) are installed to enable ultrafast live-cell 3D, simultaneous widefield imaging at diffraction-limited resolution with extremely high temporal resolution and rapid, multichannel 3D structured illumination super resolution.
System specifications
- Zen Blue 3.10 software deconvolves using GPU on dedicated processing workstation.
- Piezo controlled Fast Z-axis system - micron range
- Focus maintenance - Definite Focus System
- TIRF illumination module with 60X 1.49 TIRF lens
- STORM super resolution SMLM setup
- 40X/1.2 N.A. silicone oil immersion objective for matching of refractive indexes during deeper live cell imaging
- Environmental control (heating, CO2) -- please book extra time for heating and cooling of instrument. If you let us know ahead of time verbally, we'd avoid billing that portion of the booking out.
- Real-time job DIRECT processing on workstation linked directly to the controller PC.
Objectives :
| Objectives | WD |
| FL Plan-Neofluoar 10x/0.3DRY | 5.3 mm |
| Plan-Apochromat 20x/0.8DRY | 0.61 mm |
| LD LCI Plan-Apochromat 25x/0.8IMM CORR DIC W/SIL/GLY/OIL | 0.57 mm |
| LD LCI Plan-Apochromat 40x/1.2IMM KORR INF/0.15-0.19 W/Sil/Gly | 0.41 mm |
| Plan-Apochromat 63x/1.4 | 0.19 mm |
| Inf. Plan-Apochromat 63x/1.46 | 0.10 mm |
Lasers:
405nm(50mW); adjustables: 488nm(500mW), 561nm(500mW); 640nm (500mW)
Detection:
Duolink Hamamatsu ORCA Fusion BT sCMOS qe~95%
Emission filters:
DAPI or equivalent dye 420-480 nm
AlexaFluor488 or equivalent dye 495-550 nm
AlexaFluor568, mCherry or equivalent dye 575-620 nm
Cy5/AlexaFluor647 or equivalent dye 660-720 nm
Dualink signal splitter options: LP650, BP490-560+LP640
Funding:This microscope was funded by a NIH S10 Biomedical Research Support Shared Instrumentation Grant (Dr. Anne Villeneuve). Installation date: 09/2024
Required Acknowledgement:The following acknowledgement is required when publishing data obtained with this instrument:"The project described was supported, in part, by Award Number 1S10OD034400-01A1 from the National Center for Research Resources (NCRR). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NCRR or the National Institutes of Health."
CSIF RRID: SCR_017787