RESERVE THIS MICROSCOPE
The microscope is fully enclosed for heating and environmental control with media perfusion capabilities as well as CO2 control.
Applications:
- Imaging of weak fluorescence signals with high sensitivity AiryScan mode
- Ideal for live cells imaging using the Airyscan Multiplex
- Spectral Imaging, Unmixing
- Excitation Fingerprinting
- Super Resolution imagining with AirScan (1.7X improvement over diffraction limited imaging)
- FRET, FRAP and FLIP
- Photoactivation/ -conversion
System specifications:
- Inverted Zeiss Axio Observer 7 microscope with Definite Focus 2
- Scanning Stage with 130nmm X 100nm travel range, max. speed of 25mm/s and 0.1um resolution
- 34 spectral detection channels via GaAsP photodetector plus two PMTs
- Spectral unmixing, spectral fingerprinting and photon counting
- InVivo Scientific enclosure w/ environmental control (temperature and CO2)
- Z piezo stage
- Detection from 390 to 750 nm freely configurable
Software:
- Zen Black
- Zen Blue
Zen lite free download page here:
http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen-lite.html#downloads
Objectives
|
Correction |
Magnification |
NA |
Immersion |
working distance |
|
PLAN APO |
10x |
0.45 |
air |
2.0 mm |
|
PLAN APO |
20x |
0.8 |
air |
0.55 mm |
|
LD LCI PLAN APO |
40x |
1.2 |
Gly, Water, Silicone |
0.41 mm |
|
PLAN APO |
63x |
1.4 |
oil |
0.19 mm |
|
LD LCI PLAN APO |
25x |
0.8 |
Oil, Gly, Water, Silicone |
0.57 mm |
Laser lines (confocal):
- Diode laser 405nm
- Diode laser 445nm
- Diode laser 488nm
- Diode laser 514nm
- DPSS laser 561nm
- Diode laser 639nm
Light Source and filters (widefield):
Colibri 7 type RGB-UV equipped with 4 solid state LED lamps:
- 385nm
- 475nm
- 555nm
- 630nm
Filter set 90 HE LED:
- Beam splitter : QBS 405 + 493 + 575 + 653
- Emission filter : QBP 425/30 + 514/30 + 592/30 + 709/100.
Laser Power measure out of the 20X Dry (in nW) - ???
| Laser Wavelength in nm | 1% | 10% | 100% |
|---|---|---|---|
| 405 | |||
| 458 | |||
| 488 | |||
| 514 | |||
| 561 | |||
| 633 |
Detection:
- 2 32-channel gallium arsenide phosphide photomultiplier tube (GaAsP-PMT) for light sensitivity/spectra unmixing and Airyscan.
- 2 standard multialkali photomultiplier tubes (PMT).
- 1 T-PMT for transmitted light
- 1 AxioCam 503 mono for widefield imaging
Off-hour credits:
- Labs that use the following microscopes: Zeiss LSM780 or Nikon-SDC for 25 or more hours per month, during OFF-HOURS will be charged $30/hour for all use of that microscope.
- Labs that use the Zeiss LSM780 or Nikon SDC, live cell image system 75 or more hours per month, during OFF-HOURS will be charged at $20/hour for usage. This rate is in recognition of the high number of hours required for continuous overnight and weekend imaging inherent in the use of these microscopes for live cell imaging.
To qualify for these rates you must have after-hours access to the facility (i.e. be an experienced, competent user) and must use the confocal/2 photon between 5:00 pm and 8:00 am M-F, or any time on weekends. Reserved but unused time does not qualify for these discounts. Please speak with the facility Director for more information.
Funding:
This microscope was funded by the Wu Tsai Neurosciences Institute
