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Inverted Zeiss LSM 980 laser scanning confocal microscope with Airyscan 2

RESERVE THIS MICROSCOPE


The microscope is fully enclosed for heating and environmental control with media perfusion capabilities as well as CO2 control.

Applications:

  • Imaging of weak fluorescence signals with high sensitivity AiryScan mode
  • Ideal for live cells imaging using the Airyscan Multiplex
  • Spectral Imaging, Unmixing
  • Excitation Fingerprinting
  • Super Resolution imagining with AirScan (1.7X improvement over diffraction limited imaging)
  • FRET, FRAP and FLIP
  • Photoactivation/ -conversion

System specifications:

  • Inverted Zeiss Axio Observer 7 microscope with Definite Focus 2
  • Scanning Stage with 130nmm X 100nm travel range, max. speed of 25mm/s and 0.1um resolution
  • 34 spectral detection channels via GaAsP photodetector plus two PMTs
  • Spectral unmixing, spectral fingerprinting and photon counting
  • InVivo Scientific enclosure w/ environmental control (temperature and CO2)
  • Z piezo stage
  • Detection from 390 to 750 nm freely configurable

Software:

  • Zen Black
  • Zen Blue

Zen lite free download page here:

http://www.zeiss.com/microscopy/en_us/products/microscope-software/zen-lite.html#downloads

Objectives 

Correction

Magnification

NA

Immersion

working distance

PLAN APO

10x

0.45

air

2.0 mm

PLAN APO

20x

0.8

air

0.55 mm

LD LCI PLAN APO

40x

1.2

Gly, Water, Silicone

0.41 mm

PLAN APO

63x

1.4

oil

0.19 mm

LD LCI PLAN APO

25x

0.8

Oil, Gly, Water, Silicone

0.57 mm

Laser lines (confocal):

  • Diode laser 405nm
  • Diode laser 445nm
  • Diode laser 488nm
  • Diode laser 514nm
  • DPSS laser 561nm
  • Diode laser 639nm

Light Source and filters (widefield):

Colibri 7 type RGB-UV equipped with 4 solid state LED lamps:

  • 385nm
  • 475nm
  • 555nm
  • 630nm

Filter set 90 HE LED:

  • Beam splitter : QBS 405 + 493 + 575 + 653
  • Emission filter : QBP 425/30 + 514/30 + 592/30 + 709/100.

Laser Power measure out of the 20X Dry (in nW) - ???

Laser Wavelength in nm 1% 10% 100%
405      
458      
488      
514      
561      
633      

 

Detection:

  • 2 32-channel gallium arsenide phosphide photomultiplier tube (GaAsP-PMT) for light sensitivity/spectra unmixing and Airyscan.
  • 2 standard multialkali photomultiplier tubes (PMT).
  • 1 T-PMT for transmitted light
  • 1 AxioCam 503 mono for widefield imaging

Off-hour credits:

  • Labs that use the following microscopes: Zeiss LSM780 or Nikon-SDC for 25 or more hours per month, during OFF-HOURS will be charged $30/hour for all use of that microscope.
  • Labs that use the Zeiss LSM780 or Nikon SDC, live cell image system 75 or more hours per month, during OFF-HOURS will be charged at $20/hour for usage. This rate is in recognition of the high number of hours required for continuous overnight and weekend imaging inherent in the use of these microscopes for live cell imaging.

To qualify for these rates you must have after-hours access to the facility (i.e. be an experienced, competent user) and must use the confocal/2 photon between 5:00 pm and 8:00 am M-F, or any time on weekends. Reserved but unused time does not qualify for these discounts. Please speak with the facility Director for more information.

Funding:

This microscope was funded by the Wu Tsai Neurosciences Institute